Pharmacy Practice MCQ, in this article we will solve, Practice MCQ under subject Microbiology. Read following article for your reference.

Phase Contrast Microscopy, Dark Field Microscopy And Electron Microscopy » PHARMACAREERS

  1. Who invented phase contrast microscopy?
    • A) Albert Einstein
    • B) Frits Zernike
    • C) Robert Hooke
    • D) Antonie van Leeuwenhoek
  2. What is the primary advantage of phase contrast microscopy?
    • A) High magnification
    • B) Observing live cells without staining
    • C) High resolution
    • D) Low cost
  3. What type of light source is typically used in phase contrast microscopy?
    • A) LED
    • B) Fluorescent
    • C) Tungsten-halogen lamp
    • D) Laser
  4. What does the phase plate in phase contrast microscopy do?
    • A) Magnifies the image
    • B) Converts phase shifts into brightness changes
    • C) Stains the specimen
    • D) Filters the light
  5. In positive phase contrast, how does the specimen appear?
    • A) Brighter against a dark background
    • B) Darker against a bright background
    • C) Colorful
    • D) Transparent
  1. What is the main feature of dark field microscopy?
    • A) Uses electrons instead of light
    • B) Makes tiny, clear objects stand out against a dark background
    • C) Requires staining of specimens
    • D) Uses polarized light
  2. Which of the following is a key application of dark field microscopy?
    • A) Observing stained specimens
    • B) Viewing live, unstained cells
    • C) Studying fluorescent samples
    • D) Imaging metallic surfaces
  3. What type of condenser is used in dark field microscopy?
    • A) Bright field condenser
    • B) Phase contrast condenser
    • C) Dark field condenser
    • D) Fluorescent condenser
  4. What is the primary advantage of dark field microscopy?
    • A) High resolution
    • B) High contrast for transparent specimens
    • C) Low cost
    • D) Easy to use
  5. In dark field microscopy, how does the specimen appear?
    • A) Dark against a bright background
    • B) Bright against a dark background
    • C) Colorful
    • D) Transparent
  1. What is the main difference between electron microscopy and light microscopy?
    • A) Electron microscopy uses light
    • B) Electron microscopy uses electrons
    • C) Electron microscopy uses sound waves
    • D) Electron microscopy uses magnetic fields
  2. Which type of electron microscopy provides detailed surface images?
    • A) Transmission Electron Microscopy (TEM)
    • B) Scanning Electron Microscopy (SEM)
    • C) Phase Contrast Microscopy
    • D) Dark Field Microscopy
  3. What is the primary advantage of electron microscopy?
    • A) Observing live cells
    • B) High resolution and detailed images
    • C) Low cost
    • D) Easy to use
  4. What is used as the source of illumination in electron microscopy?
    • A) Light
    • B) Electrons
    • C) X-rays
    • D) Gamma rays
  5. Which of the following is a limitation of electron microscopy?
    • A) Low resolution
    • B) Cannot observe live specimens
    • C) Requires staining
    • D) Low magnification
  1. Which microscopy technique is best for observing live, unstained cells?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Fluorescence Microscopy
  2. Which microscopy technique uses a phase plate?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Bright Field Microscopy
  3. Which microscopy technique is known for providing high contrast images of transparent specimens?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Polarized Light Microscopy
  4. Which microscopy technique uses electrons instead of light to form images?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Fluorescence Microscopy
  5. Which microscopy technique is most suitable for studying the internal structure of cells?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Transmission Electron Microscopy (TEM)
    • D) Scanning Electron Microscopy (SEM)
  6. Which microscopy technique enhances the contrast of transparent specimens by converting phase shifts into brightness changes?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Fluorescence Microscopy
  7. Which microscopy technique is commonly used to observe bacteria and other microorganisms?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Bright Field Microscopy
  8. Which microscopy technique requires a special condenser to create a dark background?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Fluorescence Microscopy
  9. Which microscopy technique can achieve the highest magnification and resolution?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Bright Field Microscopy
  10. Which microscopy technique is best for observing the surface details of a specimen?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Scanning Electron Microscopy (SEM)
    • D) Transmission Electron Microscopy (TEM)
  11. Which microscopy technique uses a tungsten-halogen lamp as a light source?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Fluorescence Microscopy
  12. Which microscopy technique is used to observe the fine details of cell organelles?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Transmission Electron Microscopy (TEM)
    • D) Scanning Electron Microscopy (SEM)
  13. Which microscopy technique is best for observing unstained, transparent specimens?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Fluorescence Microscopy
  14. Which microscopy technique uses a phase annulus in the condenser?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Electron Microscopy
    • D) Bright Field Microscopy
  15. Which microscopy technique is used to observe the detailed surface structure of materials?
    • A) Phase Contrast Microscopy
    • B) Dark Field Microscopy
    • C) Scanning Electron Microscopy (SEM)
    • D) Transmission Electron Microscopy (TEM)

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